Acme

• Acme Corporation, in the Road Runner/Wile E. Coyote animated shorts, later a generic fictional company name
• ACME Detective Agency, in the Carmen Sandiego video game series
• Acme and Septimius, a fictional couple in Catullus 45, a poem by Roman poet Catullus
• Acme (band), a Japanese metalcore band
• Acme (album), by the Jon Spencer Blues Explosion, 1998
• Acme (card game), a patience or card solitaire

Aircraft and vehicle manufacturing companies

• Acme Aircraft Co, later Sierradyne, an American aircraft manufacturer in Torrance, California
• Acme Aircraft Corporation, an American aircraft manufacturer in Rockford, Illinois
• Acme, manufacturer of the Acme automobile, an American early motor car
• Acme Motor Co, an English motorcycle manufacturer
• Acme Motor Truck Company, an American early motor truck manufacturer
• Air Craft Marine Engineering (ACME), a short-lived American aircraft manufacturer

Other manufacturing companies

• Acme Boots, an American manufacturer of boots
• Acme Brick, an American manufacturer of brick and masonry-related construction products
• ACME Laboratories, a pharmaceutical company based in Bangladesh
• Acme Packet, an American communication components manufacturer
• Acme Tackle Company, an American producer of fishing tackle
• Acme United Corporation, an American supplier of cutting, measuring and safety products
• Acme Whistles, formed as J Hudson & Co

Arts, entertainment and media companies

• ACME Comedy Theatre, Los Angeles, California, US
• ACME Comics & Collectibles, a store in Sioux City, Iowa, US
• ACME Communications, an American broadcasting company
• ACME Newspictures, an American news agency 1923–1952
• Acme Press, later Acme Comics, a British comic book publisher 1986–1995
• Acme, a moniker that preceded Rockstar North, a video game developer

Other companies

• Acme Bread Company, a bakery in Berkeley, California, US
• Acme Fresh Market, a chain of grocery stores in Ohio, US
• Acme Markets, an American supermarket chain
• Acme Naming, a company founded by Andrea Carla Michaels
• Acme Tools, or Acme Electric Motor, Inc., an American tool and equipment retail business
• Acme Truck Line, a transportation service based in Gretna, Louisiana, US
• Acme Space, an English architecture firm
• acme.com, and ACME Laboratories, owned by computer programmer Jef Poskanzer

Organizations

• Advisory Committee on Mathematics Education, of the Royal Society and the Joint Mathematical Council
• Acme Studios, a London charity that provides studio and living space for visual artists

• Acme (enslaved woman) (died 5 BC), Jewish slave and maid
• Andrea Carla Michaels, nicknamed ACME, American crossword puzzle constructor

Canada

• Acme, Alberta, a village

United States

• Acme, Indiana, an unincorporated town
• Acme, Kansas, an unincorporated community
• Acme, Louisiana, an unincorporated community
• Acme, Michigan, an unincorporated community
• Acme Township, Michigan
• Acme, North Carolina, an unincorporated community
• Acme Township, Hettinger County, North Dakota
• Acme, Oklahoma, a ghost town
• Acme, Pennsylvania, an unincorporated community
• Acme, Texas, a ghost town
• Acme, Washington, a census-designated place
• Acme, West Virginia, an unincorporated community

• Acme (computer virus)
• ACME (health software)
• Acme (text editor)
• Acme thread form, a screw thread profile
• Acme zone, a biostratigraphic zone
• Arginine catabolic mobile element, a mobile genetic element of Staphylococcus
• Automatic Certificate Management Environment, a communications protocol
• Summit or acme, a topographic term
• ACME Laboratories, an open source software host of Jef Poskanzer
• Automatic Certificate Management Environment

Ships

• USS Acme, the name of two ships
• USS Abarenda (IX-131), originally named SS Acme, a storage tanker
• Acme (1876), a Australian schooner
• Acme (shipentine), launched for Standard Oil in 1901
• Acme (steamboat), operated in Washington state 1899–1910

Vehicles

• Acme (automobile), an early American automobile
• Acme motorcycle (1911–1913), by the Acme Motor & Engineering Company of Melbourne, Australia
• Acme motorcycle (1915–1917), assembled by EMY Ready in Tasmania, Australia
• Acme motorcycle (1939–1949), assembled and marketed by Bennett & Wood of Sydney, Australia

• Acme Building, in Billings, Montana, US
• Acme Farm Supply Building, in Nashville, Tennessee, US
• ACME Comic Con, a fan convention in Glasgow, Scotland
• Australian Centre for the Moving Image, a museum in Melbourne, Australia